The invention of PCR technology and the history of viral nucleic acid detection

Abstract: Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. The inventor of the PCR technique was Dr. Kary Mullis. He won the 1993 Nobel Prize in Chemistry for his 1983 invention of PCR. In 2015, Richard Rothschild obtained a US patent for the detection of viral nucleic acid using PCR technology. From 2020 to 2022, all countries in the world have used nucleic acid detection methods to identify the infection of 2019 novel coronavirus (2019-ncov), but the error is large and the harm is serious.

Key words: PCR Kelly Mullis 2019-nCoV COVID-19 nucleic acid testing

Introduction to PCR (polymerase chain reaction) technology

PCR, which stands for Polymerase Chain Reaction (PCR), is a molecular biological technique used to amplify specific DNA fragments. PCR can be regarded as a specific DNA replication in vitro. See the Baidu Encyclopedia entry "Polymerase chain reaction" and reference [1] for details.

PCR consists of a cycle of denaturation at high temperature (95 degrees), annealing at low temperature, and extension at appropriate temperature (60-70 degrees), which allows the target DNA to be rapidly amplified.

PCR has the characteristics of high specificity, high sensitivity, simple operation and time saving. The greatest feature of PCR is that it can greatly increase the amount of DNA. Despite its power, PCR has limitations. The length of DNA fragments it is able to replicate is limited and cannot be particularly large.

PC can be used not only in basic research such as gene isolation, cloning and nucleic acid sequence analysis, but also in disease diagnosis and DNA detection. For example, whether it is an ancient creature in a fossil, the remains of a historical figure, or the hair, skin, or blood left by a murderer in a murder decades ago, any bit of DNA that can be isolated can be amplified by PCR and compared. This is the power of "trace evidence" in scientific crime solving.

Assays that apply PCR are called PCR assays.

Kary Mullis, inventor of PCR

The inventor of the PCR technique was Dr. Kary Mullis.

Kary Banks Mullis (December 1944 - August 7, 2019) was an American chemist who won the 1993 Nobel Prize in Chemistry for his invention of the polymerase chain reaction (PCR) method for highly efficient replication of DNA fragments.

Kelly Mullis was born in 1944 in North Carolina, USA. He graduated from Georgia Institute of Technology in 1966 and received a PhD in biochemistry from the University of California, Berkeley in 1973.

In 1983, Dr. Kelly Mullis proposed the idea of polymerase chain reaction.

In 1985, Kelly Mullis invented the polymerase chain reaction, or simple DNA amplification method. This means the birth of PCR technology.

On October 25, 1985, Kelly Mullis applied for a patent for PCR. On July 28, 1987, his application was granted (patent number 4,683,202).

The first academic paper on PCR was published by Kelly Mullis et al. in the journal Science on December 20, 1985.

Kelly Mullis giving a special presentation at Cold Spring Harbor Laboratory in New York in May 1986. Since then, the world began to learn and apply PCR methods.

1993 Kelly Mullis and Michael Smith shared the Nobel Prize in Chemistry for the invention of PCR.

After winning the Nobel Prize, Mullis said he thought of the breakthrough while driving from Berkeley to Mendocino. On a dark road, he "had a eureka moment to solve the most vexing problem in DNA chemistry". This is the inspiration for scientists

Viral nucleic acid testing and Rothschild's assay patent

Experts in the United States applied PCR technology to develop a Nucleic Acid Test method to detect the virus. Nucleic acid testing is only one of many PCR tests.

Wu Shouquan, an attending physician at West China Hospital of Sichuan University, said PCR can be used to amplify DNA, so that a small amount of DNA can be detected. Some viruses, whose nucleic acid is DNA, need to be detected using this method. PCR is a method of detecting DNA. For example, this method is used to quantify hepatitis B virus DNA. The purpose of DNA examination is to diagnose diseases. If the DNA of the pathogen can be detected, it means that the body of the tested person has this pathogen. The other is to determine how much virus is replicating, and thus how severe the disease is or how effective treatment is.

For nucleic acid test, it needs to go through five steps: sampling, sample retention, retention, nucleic acid extraction, and computer detection.

In 2015, the Rothschilds were the first to file a patent for a COVID-19 test. Both the inventor and the owner of the patent were Richard A Rothchild; The patent application was dated October 13, 2015. A year later, on October 13, 2016, the U.S. Patent Office granted the patent application. The technique used by Richard Rothschild was the same PCR technique developed by Kelly Mullis.

Note: In the winter of 2019, the novel coronavirus 2019-ncov (also known as SARS-CoV-2) emerged in the United States and China. On February 11, 2020, Tedros Adhanom Ghebreyesus, Director-General of the World Health Organization (WHO), announced in Geneva, Switzerland that the new coronavirus infection pneumonia is named COVID-19. However, Richard Rothschild used the name COVID-19 in his 2015 patent application, see references [2], [3] for details. This shows that the pre-manufacturing of the novel coronavirus and Ymeows, the release of the novel coronavirus in 2019, and the nucleic acid test in 2020 were all planned by the Freemasons and the Rothschilds.

Dr. Kelly Mullis, the father of PCR, opposed the use of PCR technology for virus testing. "The PCR test is not a reliable test for the virus," he said. See ref. [4] for details.

On August 7, 2019, Nobel laureate Charles Mullis died of illness at the age of 74. Anti-virus activists in the United States suspect that Kelly Mullis was poisoned by nucleic acid interests.

The vast majority of those who tested positive were not infected with the virus

In 2015, a nucleic acid test developed by Richard Rothschild was patented in the United States. Due to the vigorous promotion by the United States CDC (Center for Disease Control and Prevention), WTO (World Health Organization) and mainstream media, since the outbreak of SARS-CoV-2 in the spring of 2020, all countries in the world have used nucleic acid testing to identify patients with SARS-CoV-2 infection.

In 2020 and 2021, health authorities around the world were convinced that anyone who tested positive for the virus was infected. In ancient China, the people infected with COVID-19 are divided into "confirmed cases" and "asymptomatic cases." "Asymptomatic infection cases" accounted for about 95% of the nucleic acid test positive; Shanghai accounted for 98%, see ref [5] for details.

Since January 2022, Omicron, a variant of the novel coronavirus 2019-nCoV, has been raging around the world. Soon, Omicron completely "eliminated" other variants of 2019-nCoV. From the summer of 2022, many countries, including the United States, the United Kingdom, and Canada, have determined that "asymptomatic infected persons" are healthy people without disease; Only the ancient state of China isolated and treated "asymptomatic infected persons" as disease patients.

In ancient China, "nucleic acid testing" has become a big movement throughout the country and participated by the whole people.

FDA documentation acknowledges that PCR tests for the novel coronavirus 2019-nCoV were developed, tested, and calibrated without the availability of isolated 2019-nCoV samples. This effectively acknowledges that the US PCR tests for something other than the isolated 2019-nCoV, as detailed in Ethan Huff's article on the NewsTarget website (http://NewsTarget.com) on August 1, 2021, ref [6].

On December 8, 2021, the NewsTarget website (http://NewsTarget.com) published the article "PCR Tests and the Rise of Disease Panic" in Chinese. The author is the news editor of the website. See reference [7]. The article says:

Investigating whether a person suffers from a certain disease is like investigating whether the suspect is the culprit in the case. Just as the detection of a suspect's DNA at a crime scene does not prove that the suspect committed the crime, the detection of viral DNA in someone does not prove that the person tested has a disease from which the virus was infected.

The news editor of the website used the facts in the United States to explain that the vast majority of those who tested positive for viral nucleic acid were asymptomatic, not COVID-19 patients, but normal healthy people.

Non-mainstream media in the US and UK, mainly websites, have published many articles about serious problems with nucleic acid testing.

Our next article will elaborate on the relationship between positive nucleic acid test results and viral infection and COVID-19.

The COVID-19 pandemic, which occurred in 2019, is the longest, most harmful and most far-reaching malignant event in human history planned, created and carried out by groups against humanity. "Nucleic acid detection" is the third stage of this event segment. The first stage was the release of 2019-nCoV and the outbreak of SARS-CoV-2. The second stage is to carry out COVID-19 Yimeow vaccination and promote it to people around the world.

Anything that happens in human society will end. Now, except for ancient China, the third phase of the COVID-19 epidemic "nucleic acid testing" in all countries in the world is over. Therefore, the movement of "nucleic acid detection" in ancient China must also end. The end of the "nucleic acid testing" campaign in ancient China is the end of the COVID-19 pandemic in the world.